|
KMID : 0644120110240020013
|
|
Journal of Oriental Gynecology
2011 Volume.24 No. 2 p.13 ~ p.32
|
|
|
Effects of Danchisoyo-san on UVB-induced Cell Damage and Gene Expression in Dermal Fibroblast
|
|
Lim Hyun-Jung
Yoo Dong-Youl
|
|
|
Abstract
|
|
|
Objectives:This study was performed to elucidate the effects of Danchisoyo-san (DS) on cell damage and gene expression in UVB-exposed dermal fibroblast.
Methods:To demonstrate the inhibitory effects of DS on aging of the skin, we used human dermal fibroblast(F6) and UVB light(30 mJ/㎝²) was used to damage to dermal fibroblast. We measured the nitrite production, LDH release, and gene expression in UVB-irradiated dermal fibroblast to elucidate the actionmechanism of DS. Also, we evaluated the amount of increased PICP, TIMP-1 in dermal fibroblast. PICP, TIMP-1 concentration was measured using EIA kit, and gene expression (MMP-1, procollagen, c-fos, c-jun, NF-kB, Bcl-2, Bcl-xL, iNOS) were determined using real-time PCR.
Results:1. DS inhibited LDH-release, nitrite production in UVB-irradiated dermal fibroblast. 2. DS suppressed the gene expression of MMP-1 in UVB-irradiated dermal fibroblast. 3. DS increased the gene expression of procollagen in UVB-iradiated dermal fibroblast. 4. DS suppressed the gene expression of c-jun, c-fos, NF-kB, iNOS in UVBirradiated dermal fibroblast. 5. DS increased the gene expression of Bcl-2 in UVB-iradiated dermal fibroblast. 6. DS increased the cell proliferation of dermal fibroblast.
Conclusions :From the results, we concluded DS increases the cell proliferation and collagen synthesis in dermal fibroblast. So we suggest that DS has the antiwrinkle effects.
|
|
|
KEYWORD
|
|
|
Danchisoyo-san(DS), Dermal fibroblast, UVB, Collagen, Antiwrinkle effects, MMPs
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|